CHINA JOURNAL OF LEPROSY AND SKIN DISEASES
(Quarterly) Volume 17, Number 2,June 2001
①Detection of antibodies for IgG,IgM and IgA classes against whole Streptococcus hemolyticus
in sera from patients with psoriasis and their significance
②A preliminary study on the expression of CD44s in some skin tumors
③The expression of vascular endothelial growth factor and the density of tumoral microvessels
in non-invasive and invasive basal cell carcinoma
④Determination of serum SIL-2R level and its clinical significance in syphilitic patients
⑤The impact of ceramide on differentiation of cultured keratinocytes
⑥Phaeohyphomycosis caused by Cladosporium herbarum: the first case report in China
⑦Primary Vibrio vulnificus infection: A report of 12 cases
⑧Analysis on papules para-penis ferenulum in 250 cases
⑨Epidemiologic trends of syphilis in Shenzhen: 1983~1999
Chen Xiaohong,Wu Qinxue, Liu Xunquan. Institute of Dermatology, Chinese Academy of Medical Sciences and Peking Union Medical College, Nanjing, 210042
[Abstract] Objective: To determine antibodies for IgG, IgM and IgA classes against whole Streptococcus hemolytic-us (WSC) in sera from patients with psoriasis and to correlate the antibodies with their significance. Methods: Antibodies against WSC antigens were detected with ELISA in patients with psoriasis and normal controls. Results: The titers of the antibodies against WSC antigens were different in all types of psoriasis and the distribution of antibodies showed of the antibodies against WSC antigens were different in all types of psoriasis and the distribution of antibodies showed the pattern of PG>PV>PE>PA>PP. The level of IgM antibody was significantly different between all types of psoriasis and normal controls. There was a significant difference in the level of IgG Antibody between PG and other types of psoriasis (PE, PA and PP). Conclusion: The results suggest that Streptococcus hemolytic-us might play an important role in the pathogenesis of psoriasis, particularly for guttate psoriasis.
[Key words] psoriasis; Streptococcal hemolytic-us; immunoglobulin
A preliminary study on the expression of CD44s in some skin tumors
Li Yuanhong, He Chundi, Chen Hongduo. Department of Dermatology, First Hospital of China Medical University, Shenyang, 110001
[Abstract] Objective: To investigate the expression of CD44s in some skin tumors. Methods: Immunohistochemical method was used to detect the expression of CD44s, V4/5 and V6 in squamous cell carcinoma (SCC), basal cell carcinoma (BCC), mycosis fungoides (MF), Bowen’s disease, malignant melanoma (MM), seborrheic keratosis, melanocytic naevus and normal skin. Results: In SCC and BCC the cancer nest was more nearer to the epidemis the expression of CD44s was more stronger. The expression of CD44s in MM and naevus was similar to that in SCC and BCC. In other skin tumors the expression of CD44s was as the same as the normal skin. Conclusion: The expression of CD44s was correlated with the location of the skin cancer nest and there is no association between the expression of CD44s and the malignancy of naevus cells.
[Key words] CD44S; CD44V
Deng Liehua, Luo Yuwu, Zhao Gang, et al. Department of Dermatology, The First Affliated Hospital of Medical College of Jinan University, Guangzhou, 510630
[Abstract] Objective: To investigate the expression of vascular endothelial growth factor (VEGF), the density of tumoral microvessels (TMVD), and their significance in non-invasive basal cell carcinoma (NIBCC) and invasive basal cell carcinoma (IBCC). Methods: We used SP immunohistochemical staining for observing VEGF and Von-Willebrand factor in NIBCC and IBCCs. Results: There were no statistically significant differences in the moderate-level expression of VEGF between NIBCC and IBCC (p=0.189). The level of TMVD in IBCC was higher Than that in NIBCC (p=0.019). The expression of VEGF was positively correlated both in NIBCC and IBCCs(rs1=0.667, p=0.003; rs2=0.582, p=0.014). Conclusion: Based on this study VEGF might not be taken as an indicator of the malignancy in BCC, while TMVD is a good indicator of the malignancy in BCC, while TMVD is a good indicator in assessing the invasiveness of BCC.
[Key words] basal cell carcinoma; vascular endothelial growth factor; Von-Willebrand factor; tumoral microvascular density
Determination of serum SIL-2R level and its clinical significance in syphilitic patients
Lin Linlin, Wu Huizhen, Chen Yunqin, et al. Department of Dermatology, First Affiliated Hospital Wenzhou Medical college, Wenzhou, 325000
[Abstract] Objective: To determine the serum SIL-2R level and its clinical significance in syphilitic patients. Methods: The serum SIL-2R level and RPR change before and after benzylpenicillin therapy in patients with syphilis were determined with polyclonal antibody sandwich ELISA. The correlation of serum SIL-2R level with different stages of syphilis was analyzed. Results: The serum SIL-2R level was higher in all stages of syphilis than that in control group (p<0.001) and was higher in both secondary and latent syphilis than that in primary syphilis (p<0.01). There was no significant difference in serum SIL-2R level between secondary and latent syphilis (p>0.05). The titer of serum SIL-2R and RPR decreased in all stages of syphilis after effective treatment. Compared with control group, the serum SIL-2R level in secondary and latent syphilis was still higher (p<0.05). Conclusion: The continual dynamic monitoring of SIL-2R level for the same patient could be used for evaluating the activity of illness and the therapeutic effect.
[Key words] syphilis; SIL-2R
The impact of ceramide on differentiation of cultured keratinocytes
Sun Ling, Zhao Ye. Department of Dermatology, Second Affiliated Hospital, Dalian Medical University, Dalian, 116023
[Abstract] Objective: To investigate the impact of ceramide on the differentiation of keratinocytes cultured in serum-free medium. Methods: Two kinds of ceramide (C2 and C6) were selected and added into serum-free media with keratinocyte culture. In 0,3,6,12 and 24 hour after adding ceramides, total RNA was abstracted to detect the expression of specific keratinocyte differentiation markers: TGasel, IVL, KRT1 KRT10, etc. by RT-PCR method. Results: The expression of TGasel mRNA and IVL Mrna was most significant in 12 hours in the media containing C2 ceramide, and the levels were 5.7 times and 5.2 times than that in the control media without C2 ceramide in 12 and 24 hours, respectively. The expression of KRT1 Mrna and KRT10 Mrna in the media containing C2 ceramide was also increased but did not reach a significant level. The expression of Tgasel Mrna and IVL Mrna in the media containing C6 ceramide was similar to the media containing C2 ceramide, and was 3.3 times and 3.5 times than that in control media in 24 hours. However, the expression of KRT1 Mrna and KRT10 mRNA was significantly higher in the media containing C6 ceramide than in the media containing C2 ceramide, with 10 in the former and 5.4 times in the later. Conclusion: Ceramide could significantly increase the expression of specific differentiation markers (protein mRNA) of keratinocytes cultured in serum-free media and could induce the differentiation of keratinocyte.
[Key words] ceramide; keratinocyte; transglutaminasel; involucrin; keratin
Phaeohyphomycosis caused by Cladosporium herbarum: the first case report in China
Zhao Wei, Kuang Xiangzong, Li Hongguo, et al. Department of Dermatology, 201 Hospital of P.L.A. Liaoyang, Liaoning, 111000
[Abstract] Objective: To report a case of phaeohyphomycosis caused by Cladosporium herbarum. Methods: Apart from clinical profile, PAS stain method was used to identify the morphologic characteristics of the fungus. Results: The species of Cladosporium herbarum was identified from the patient with verrucous nodule in face. Conclusion: The case reported here was the first case with primary Cladosporium herbarum infection in China.
[Key words] Cladosporium herbarum; phaeohyphomycosis
Primary Vibrio vulnificus infection: A report of 12 cases
Li Bingxu, Wu Huizhen, Lu Zhongqiu, et al. First Hospital of Wenzhou Medical College, Wenzhou, 325000
[Abstract] Objective: To analyze 12 cases with primary Vibrio vulnificus infection (PVVI). Methods: Clinical profiles of 12 cases with primary PVVI were collected and analyzed from 1995 to 2000. Results: There were 8 males and 4 females. The average age was 48.6 years, ranging from 40 to 67 years. Six of the 12 patients had cirrhosis and the medical history of other 6 cases was nonspecific. The onset of the disease started suddenly with fever and occurred in late summer or early autumn in most cases. The most prominent clinical presentations were painful swollen, bulla and hemorrhagic bulla in lower extremities. Vibrio vulnificus was cultured positively in bullus fluid from 2 patients. The main pathological findings were local acantholysis, separation of epidemis from dermis, degeneration of collagen fibers, vasculitis and occlusion of small arteries. Under electromicroscopy, the microorganism appeared arc like with flagellum. Eight of the 12 cases died, 1 recovered and 3 lift the hospital voluntarily. The main cause of the death was multi-organ dysfunction. Conclusion: PVVI is an unrecognized acute disease with high death rate.
[Key words] Vibrio vulnificus; infection; shock
Analysis on papules para-penis ferenulum in 250 cases
Su Jingze, Fan Min, Xi Liyan, Shanghai Hospital for Skin Diseases & STDs, Shanghai, 200050
[Abstract] Objective: To confirm whether papules para-penisb ferenulum (PPPF) is a benign entity, and not related to sexual transmission. Methods: The clinical abservation and examination, testing for pathogens, histopathological, inmunohistochemical technique and electronical microscopy were used in this study. Results: The main clinical features showed that the lesions were skin color and/ or pale, millet-size papules located in the para-ferenulum of penis in younger and middle age males. The histopathological diagnosis accorded with fibroma. The results of all pathogen tests for STDs were negative. Conclusion: It seems that PPPF is an unrecognized skin entity. It may be one of atavism.
[Key words] papules para-penis ferenulum; fibroma; condyloma acuminatum
Epidemiologic trends of syphilis in Shenzhen: 1983~1999
Hong Fuchang1, Zhou Hua1, Zeng Xuchun1, et al. Shenzhen Institute of Dermatology, Shenzhen, 518020
[Abstract] Objective: To describe the epidemiology of syphilis from 1983 to 1999 in Shenzhen. Methods: Newly reported cases of syphilis from all hospitals in Shenzhen through passive surveillance system were collected and analyzed. Results: (1) The reported yearly incidence of syphilis in the period of 1983~1994 was 1.35 per 100 000 and started to increase since 1995 onward, with 1.97 times increase every year and reached at peak in 1999(66.19/100 000); (2) Yearly incidence (18.61/100 000) was little higher in female than in male (18.53/100 000) in the past 17 years; (3) The incidence was highest in the group of 20~40 years old; with an incidence of 55.39/100 000 in the group of 20~30 years old and 31.79/100 000 in the group of 31~40 years old, (4) Congenital syphilis increased rapidly during 1996~1999; (5) The patients with secondary school educational background accounted for 77.39% and workers, government officials, businessman, servants and private businessman accounted for 86.81%, The migrants accounted for 43.65%. 48.37% of patients were unmarried and 74.76% were infected through non-marital sexual contacts. The secondary and primary syphilis accounted for 57.29% and 37.65% respectively. Conclusion: The increase of incidence of syphilis has become a major public health problem in Shenzhen and effort must be made to control the epidemic of syphilis.
[Key words] syphilis; epidemiology