CHINA JOURNAL OF LEPROSY AND SKIN DISEASES
(Quarterly) Volume 17, Number1,March 2001
①Inherited
epidermolysis bullosa-a study on molecule in basement membrane zone
②Detection
of PGL-1 antigen and S-100 protein in the diagnosis of early leprosy
⑤Study
on the etiology of vulvar itching in 452 female cases
⑦Infection
of hepatitis B virus in the patients with systemic lupus erythematosus
⑧Analysis
of patch-test results for 317 patients
Inherited
epidermolysis bullosa-a study on molecule in basement membrane zone
Wu
Yan, Zhu Xuejun. The first hospital of Peking University, Beijing, 100034
[Abstract] Objective: To study molecule in basement membrane by electron microscopy and indirect immunofluores-cence in patients with inherited epidermolysis bullosa. Methods: Electron microscopy and indirect immunofluorescence were performed on skin samples from 7 patients with epidermolysis bullosa. Results: The result of electron microscopic examination: The fissure in lamina lucida was found in one patient, for whom the diagnosis of JEB was made. The fissures under lamina densa were observed in 6 patients diagnosed as DEB. Immunofluorescence showed the expression of BPAG2 were totally absent in the JEB patient. Combined with clinical manifestations, the diagnosis of GABEB was made. This was the first case found in China. The 6 DEB cases showed different degree of changes in the expression of type VⅡ collagen in the electron microscopic exam and indirect immunofluorescence detection. Thes would help in differing DDEB from RDEB. Conclusion: Inherited epidermolysis bullosa is composed of a group of disorders. It is necessary to use electron microscopy and immunofluorescence test to classify subgroup of the disease and we recommend these two methods to be used in gene mutation detection.
[Key words] inherited epidermolysis bullosa; molecules in basement membrane zone; eletron microscopy; immunofluorescence
Detection
of PGL-1 antigen and S-100 protein in the diagnosis of early leprosy
Weng
Xiaoman, Chen Shuyuan, Long Heng, et al. Beijing Institute of Tropical Diseases,
Beijing, 100050
[Abstract] Objective: To improve the histological diagnosis of leprosy and understand the features of early leprosy based on pathogen, histology and immunology. Methods: Routine (HE, AF) and immunohistochemical stainings with monoclonal antibodies against PGL-1, S-100 protein were applied to eissue sections from 45 clinically diagnosed and suspected cases. Results: (1) Senstivity of AFB and PGL antigen detection can be significantly improved by examination of 20 or more serial sections. AFB and/or PGL were mostly found in the infiltrating cells of the subepidermal zone, intraneurium, perineurium and around blood vessels. (2) PGL antigen can be found in 10 confirmed single-lesion leprosy but AFB was detected in 7 patients. Of which, AFB in nerve for 5 patients, PGL in nerve for 6 patients and both AFB and PGL in nerve for 4 patients. (3) S-100 protein staining demonstrated that nonspectific chronic inflammation in indeterminate leprosy presents as selective perineural and/or intraneural infiltration with lymphocytes predominating. (4) Except 3 cases with unknown-number of lesions, the positive correlation between the number of lesions and immuno-pathological confirmatory rate was found in nanely 41.6% (10/24) for single lesion, 66.6% (6/9) for 2 lesions and 88.8% (8/9) for patients with≥3 lesions. Conclusions: Immuno-histological study is helpful to improve histological diagnosis of early leprosy.
[Key words] early leprosy; immuno-histochemistry study; PGL-1 antigen; S-100 protein
Sun
Zhijian1, Li Dongming2, Li Ruoyu2, et al. 1Department
of Dermatology, Shandong Provincial Hospital, Jinana, 250021; 2The
First Hospital of Beijing University, Beijing, 100034
[Abstract] Objective: To evaluate the susceptibility of Exophiala spp. Against terbinafine (TBF) according to the microdilution method proposed by NCCLS. Methods: The modified NCCLS M-26A was applieded. Sixty-six strains were studied, including 19 of E. dermatitidis , 18 of E. jeanselmei, 13 of E. monilliae, 12 of E. spinifera, 2 of E. werneckii, 1 of E. salmonis, 1 of E.pisciphila. The strains were incubated at 27℃ for 5~7 days, with final inoc-ulum size of 0.5~2.5×104 CFU/ml. To compare the difference of susceptibility of Exophiala spp, media of RPMI1640 and SDB were used during the culture, respectively. Results: The range of minimum inhibitory concentrations(MICs) was 0.004~0.5 μg/ml, and no resistant strains were detected. The MIC values of TBF to all strains were concordant when RPMI-1640 or SDB medium was used. Conclusion: The data indicated that the modified NCCLS M27A method could be applied for antifungal susceptibility testing of TBF and Exophiala spp. Which was sensitive to TBF .
[Key words] Exophiala spp; MIC; terbinafine
Zhang
Xibao, Li Ji, Li Ping, et al. Guangzhou Institute of Dermatology, Guangzhou,
510095
[Abstract] Objective: To determine the prevalence of Candida spp. Infection and yeast sensitivity to antifugal agents among 167 women with high risk behaviors for the infection of STDs. Methods: The isolates from vaginal discharge were tested by microscope and cultured. Results: The prevalence rate of Cadida spp. Was12.1% (20/167) in this group of women. The main species of yeast was still Candida albicans and the strains were sensitive to most of antifungal agents. Conclusions: All of these strains isolated were sensitivity to itraconazole, fluconazole, amphotercin B and nystatin. Some of the strains showed less sensitivity to ketoconazole and econazole and a few of strains were re-sistant to miconazole and 5-fluorocytosine.
[Key words] candida spp; high risk population; drug sensitivity
Study
on the etiology of vulvar itching in 452 female cases
Yu Meiling, LI Zhongwei, Zhou Shengji, et al. Shandong Provincial Inseitute of Dermatology and Vevereology, Jinan, 250022
[Abstract] Objective: To determine the etiology of vulvar itching among females attended to dermatovenereology clinic. Methods: Physical examination was undertaken for all patienes with vulvar itching. Swabs of vaginal dis charge were tested for PH, amine test, candida, trichomonas, and clue cells. Endocervical swabs were also taken for N. gonorrhoea, u. urealyticum culture, including EIA for chlamydia. The specimen from suspected lesions were tested for HPV and HSV, and the serological tests were carried out for syphilis. Result: The detected rate of candidiasis, nopn-gonococcal cervicitis and bacterial vaginosis was 25.44%, 20.79% and 13.50%, respectively. Vulvar eczema and trichomoniasis vaginitis were also found more frequently. Conclusion: The main causes of valvar itching in this group were sexually transmitted diseases, vulvar eczema, and genital itching.
[Key words] vulvar itching; vaginitis; cervicitis; bacterial vaginosis
Yin
Guangwen, Yu Jianbin, He Fucheng, et al. Depertment of Dermatology, The First
Affiliated Hospital of Henan Medical University, Zhenzhou, 450052
[Abstract] Objective: To determine cell immunologic status of patients with condyloma acuminatum (CA) and provide theoretical basis for immunotherapy. Methods: T-lymphocyte subpopulation of peripheral blood was determined by indirect immunofloresence and the level of serium IL-2 was measured with ELISA in 36 patients with CA and 20normal controls. Results: Decrease of CD4+ cells percentage and CD4+ / CD8+ cell ratio P＜0.05, and increase of CD8+ cells percentage were found P＜0.05, and the serum IL-2 level were lower P＜0.05 in patients with CA. Conclusion: Cellular immunity may be impaired in CA patients, and probably is one of important causes of occurrence and recurrence of CA. This study provides theoretical and experimental basis for CA immunotherapy.
[Key words] condyloma acuminatum; T-lymphocyte subpopulation; interleukin-2
Infection
of hepatitis B virus in the patients with systemic lupus erythematosus
Qiu
Ning, Chen Zhiqing, Cai Xiulin,et al. Institute of Dermatology, Chinese Academy
of Medical Sciences & Peking Union Medical College, Nanjing, 210042
[Abstract] Objective: To determine the association between systemic lupus erythematosus (SLE) and hepatitis B virus (HBV) infection. Methods: HBV serological marks were detected with ELISA in 102 patients with SLE and 95 patients with non-connective tissue diseases. Results: The prevalence of HBV infection was similar in SLE and in non-connective tissue diseases. Conclusions: There was no obvious association between SLE and HBV infection. No significant difference in clinical features and immunological indicators were found in SLE patients with positive and negative HBV.
[Key words] SLE; HBV
Analysis
of patch-test results for 317 patients
Feng
Manjun, Liu Hongwei, Li Yan, et al. Department of Dermotolgy, Provincial
People’s Hospital of Henan, Zhengzhou, 450003
[Abstract] Objective: To determine the allergens for allergic contact dermatitis including eczema. Methods: The patch test was performed on 317 patients with Bianmin standard screening patch test kit. Results: 218 out of 317 patients tested were positive (68.77%). The positive rate of eczema, dermatitis and urticaria were higher than that in patients with other skin diseases. Some patients with acne vulgaris, popular urticaria and lip hyperpigmentation also had positive patch test. Conclusions: The results showed that dermatosis such as dematits and eczema could be prevented and cured by guidance of patch test; The patch test should be performed to patients with atypic dermatosis such as acne vulgaris, popular urticaria and lip dermatitis in order to rule out the possibility of contact allergy.
[Key words] patch test; dermatitis; eczema; contact dermatitis